研究

伯明翰大学researchers develop SLAM 3D bioprinting method

来自伯明翰大学已经开发了一种用于软材料的新3D打印方法,该方法可以使人工医疗植入物的生产。

该技术被称为悬浮层添加剂制造(SLAM),使用基于雷电竞充值聚合物的水凝胶,该水凝胶含有用于自修复凝胶的颗粒。可以将液体或凝胶注入该介质中以构建3D形式。

“The hydrogel we have designed has some really intriguing properties that allow us to print soft materials in really fine detail,” explains Professor Liam Grover, leader of the study published in高级功能材料

“It has huge potential for making replacement biomaterials such as heart valves or blood vessels, or for producing biocompatible plugs, that can be used to treat bone and cartilage damage.”

使用SLAM方法创建的3D打印支架。通过伯明翰大学的照片。
使用SLAM方法创建的3D打印支架。通过伯明翰大学的照片。

SLAM 3D生物打印

该团队开发了大满贯作为替代方案Freeform Reversible Embedding of Suspended Hydrogels(Fresh),它使用凝胶形成浆液浴,并在其中注入印刷材料。根据伯明翰大学的科学家的说法,这种方法可以导致凝胶培养基中的摩擦,从而扭曲印刷过程。

SLAM使用低粘度生物聚合物将其用于自我修复的流体凝胶基质。在溶胶 - 凝胶过渡过程中引入剪切应力时,形成了这种液体凝胶,以产生纠缠的凝胶微粒网络。这与新鲜的空闲“散装”凝胶不同,在没有剪切的情况下形成聚合物网络。

通过此过程,研究人员旨在证明如何剪切或扭曲凝胶中的颗粒“使它们分开,但仍然保持它们之间的联系”,如研究中所述。这种相互作用会产生自我修复效果,从而使凝胶可以精确的细节支持印刷材料,而不会泄漏或下垂。

a)流体 - 凝胶印刷床是通过在整个溶胶 - 凝胶过渡过程中剪切冷却热琼脂糖溶液来创建的,然后将其装入合适尺寸的容器中以支撑脚手架。b)生物焦挤出在自我修复的流体床中,多个墨盒可能会挤出不同的水凝胶层,形成与预沉积的生物互联的界面,以创建多层构建体。c)交联和细胞介质诱导凝固,并为细胞支架提供代谢物。d)用去离子水释放的低剪切洗涤。图像通过伯明翰大学。

Soft materials and additive manufacturing

3D printing soft materialshave been seen as a big challenge for scientists as they require support to avoid sagging. Through SLAM tests, the team found that the agarose support bath allows for further methods of crosslinking, which includes collagen formulations.

研究人员强调这是再生医学的优势。“该方法能够成功地制造出大量,复杂,双相和相位封闭的水凝胶,这些水凝胶目前在再生医学中已广泛研究。”

“Overall, SLAM is a promising technique for producing delicate soft tissues, complex soft tissue structures, and interfaced tissues.”

使用悬浮层添加剂制造(SLAM)制造复杂的水凝胶结构雷电竞充值透明由Jessica J. Senior,Megan E. Cooke,Liam M. Grover和Alan M. Smith合着。

Fabrication of complex structures by SLAM using gellan. A) Intricate lattice prior to (left) and following extraction (right) from the fluid‐gel bed. B) T7 intervertebral disc as a CAD file (left) and demonstrating the printing of bulk structures with lateral (middle) and apical (right) views. C) Intricate bulk structure in the form of a gellan spider. D) Carotid artery as a CAD file (left) and during 3D printing (right). Image via the University of Birmingham.

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特色图显示了使用SLAM方法创建的3D打印脚手架。通过伯明翰大学的照片。